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產品名稱：Invitrogen 18009019 CALF INTESTINAL ALK. PHOSPH.
貨 號：18009019
規 格：1000 units
分 類：DNA/RNA 修飾酶
品 牌：Invitrogen
國產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態：現 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業供應Invitrogen 18009019 CALF INTESTINAL ALK. PHOSPH.常備現貨，*。主營Life Invitrogen產品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規化學試劑、培養基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3′ and 5′ phosphates from DNA and RNA.
Concentration: 20 units/μL
Applications: Dephosphorylation of 5′-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5′ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from calf intestinal mucosa.
Performance and Quality Testing: Endodeoxyribonuclease, 3′ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition: One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 μl for 10 min. at 37°C.
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